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Genome-wide Analyses of the Structural Gene Families Involved in the Legume-specific 5-Deoxyisoflavonoid Biosynthesis of Lotus japonicus

机译:全基因组分析的结构基因家族参与豆科植物特异性的日本莲的5-脱氧异黄酮类生物合成。

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摘要

A model legume Lotus japonicus (Regel) K. Larsen is one of the subjects of genome sequencing and functional genomics programs. In the course of targeted approaches to the legume genomics, we analyzed the genes encoding enzymes involved in the biosynthesis of the legume-specific 5-deoxyisoflavonoid of L. japonicus, which produces isoflavan phytoalexins on elicitor treatment. The paralogous biosynthetic genes were assigned as comprehensively as possible by biochemical experiments, similarity searches, comparison of the gene structures, and phylogenetic analyses. Among the 10 biosynthetic genes investigated, six comprise multigene families, and in many cases they form gene clusters in the chromosomes. Semi-quantitative reverse transcriptase–PCR analyses showed coordinate up-regulation of most of the genes during phytoalexin induction and complex accumulation patterns of the transcripts in different organs. Some paralogous genes exhibited similar expression specificities, suggesting their genetic redundancy. The molecular evolution of the biosynthetic genes is discussed. The results presented here provide reliable annotations of the genes and genetic markers for comparative and functional genomics of leguminous plants.
机译:模型豆科植物日本莲花(Regel)K. Larsen是基因组测序和功能基因组计划的主题之一。在针对豆类基因组学的靶向方法的过程中,我们分析了编码与日本豆类的豆类特异性5-脱氧异类黄酮生物合成有关的酶的基因,该酶在激发子处理中产生异黄酮植物抗毒素。通过生化实验,相似性搜索,基因结构比较和系统发育分析,尽可能全面地分配了同源的生物合成基因。在研究的10个生物合成基因中,有6个包含多基因家族,在许多情况下,它们在染色体中形成基因簇。半定量逆转录酶-PCR分析显示在植物抗毒素的诱导过程中大多数基因的协同上调和转录物在不同器官中的复杂积累模式。一些旁系同源基因表现出相似的表达特异性,表明它们的遗传冗余。讨论了生物合成基因的分子进化。此处提供的结果为豆科植物的比较和功能基因组学提供了可靠的基因和遗传标记注释。

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